AMPLIFICA TION OF HVR1 REGION OF HEPA TITIS C VIRUS WITH PA TIENT SPECIFIC NESTED PCR PRIMERS

Improved quantitative PCR using nested primers.

Quantitative PCR can often be improved by conducting the amplification with nested primers. First, fewer nonspecific amplification products, which could otherwise interfere with quantitation, are produced. Often, nonspecific products can be eliminated. In these cases, relatively simple nonspecific detection techniques are suitable for quantitation. In addition, nested primer PCR provides intrin...

Prevalence of Oesophagostomum bifurcum and Neca- tor americanus infections using specific PCR amplifica- tion of DNA from fecal samples

Selecting specific PCR primers with MFEprimer.

Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) ...

Single-tube nested PCR using immobilized internal primers for the identification of dengue virus serotypes.

Molecular techniques based on the detection of genomic sequences by reverse transcription (RT)-PCR, nested PCR, or real-time PCR have made possible the rapid diagnosis of dengue virus (DENV) infections, and these approaches have been accepted by clinical laboratories as the new standard method for the detection of dengue virus in acute-phase serum samples. One of these PCR-based assays, the two...

Detection and Typing of Human Papilloma Virus by Multiplex PCR with Type-Specific Primers

The primary underlying cause of cervical cancer is infection with one or more high-risk (HR) types of the human papilloma virus (HPV). Detection and typing of HPV have been commonly carried out by PCR-based assays, where HPV detection and typing are two separate procedures. Here, we present a multiplex PCR-based HPV typing assay that detects 20 HPV types (15 HR, 3 probably HR and 2 low risk) us...